SPHK1, CT (R414) (SPHK1, SPHK, SPK, Sphingosine kinase 1)

SPHK1 is a novel lipid messenger with both intracellular and extracellular functions. Intracellularly, it regulates proliferation and survival, and extracellularly, it is a ligand for EDG1 (MIM 601974). Various stimuli increase cellular levels of SPP by activation of sphingosine kinase (SPHK), the enzyme that catalyzes the phosphorylation of sphingosine. Competitive inhibitors of SPHK block formation of SPP and selectively inhibit cellular proliferation induced by a variety of factors, including platelet-derived growth factor (e.g., MIM 173430) and serum.\n\nApplications:\nSuitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA\n\nRecommended Dilution:\nELISA: 1:1,000\nWestern Blot: 1:100-500\nImmunohistochemistry: 1:50-100\nFlow Cytometry: 1:10-50\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

042227

Size

200ul

Applications

ELISA, FC, IHC, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

P Type

Pab

Purity

Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.

Isotype

IgG

References

Hengst, J.A., et al. Arch. Biochem. Biophys. 494(1):23-31(2010)\nJarman, K.E., et al. J. Biol. Chem. 285(1):483-492(2010)\nGamble, J.R., et al. Am. J. Pathol. 175(5):2217-2225(2009)

Additional Info

Human

Alternative Names

SPHK1, SPHK, SPK, Sphingosine kinase 1