SPHK1 (Sphingosine Kinase 1, SK 1, SPHK, SPK 1, SPK)

Applications:\nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nAA Sequence:\nMDPAGGPRGVLPRPCRVLVLLNPRGGKGKALQLFRSHVQPLLAEAEISFTLMLTERRNHARELVRSEELGRWDALVVMSGDGLMHEVVNGLMERPDWETAIQKPLCSLPAGSGNALAASLNHYAGYEQVTNEDLLTNCTLLLCRRLLSPMNLLSLHTASGLRLFSVLSLAWGFIADVDLESEKYRRLGEMRFTLGTFLLLAALRTYRGRLAYLPVGRVGSKTPASPVVVQQGPVDAHLVPLEEPVPSHWTVVPDEDFVLVLALLHSHLGSEMFAAPMGRCAAGVMHLFYVRAGVSRAMLLRLFLAMEKGRHMEYECPYLVYVPVVAFRLEPKDGKGVFAVDGELMVSEAVQGQVHPNYFWMVSGCVEPPPSWKPQQMPPPEEPL\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

133768

Size

100ug

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG2a,k

References

1. The sphingosine kinase 1 and S1P1 axis specifically counteracts LPS-induced IL-12p70 production in immune cells of the spleen. Schroder M, Richter C, Juan MH, Maltusch K, Giegold O, Quintini G, Pfeilschifter JM, Huwiler A, Radeke HH.Mol Immunol. 2011 Mar 22. [Epub ahead of print] 2. The role of Eag and HERG channels in cell proliferation and apoptotic cell death in SK-OV-3 ovarian cancer cell line. Asher V, Warren A, Shaw R, Sowter H, Bali A, Khan R.Cancer Cell Int. 2011 Mar 10;11:6. 3. Effect of HFE Variants on Sphingolipid Expression by SH-SY5Y Human Neuroblastoma Cells. Ali-Rahmani F, Hengst JA, Connor JR, Schengrund CL.Neurochem Res. 2011 Jan 18. [Epub ahead of print] 4. Sphingosine kinases regulate NOX2 activity via p38 MAPK-dependent translocation of S100A8/A9. Schenten V, Melchior C, Steinckwich N, Tschirhart EJ, Brechard S.J Leukoc Biol. 2011 Apr;89(4):587-96. Epub 2011 Jan 13. 5. Increased radiation sensitivity of head and neck squamous cell carcinoma with sphingosine kinase 1 inhibition. Sinha UK, Schorn VJ, Hochstim C, Chinn SB, Zhu S, Masood R.Wiley InterScience (www.interscience.wiley.com). DOI: 10.1002/ hed.21418 6. A polysaccharide, MDG-1, induces S1P1 and bFGF expression and augments survival and angiogenesis in the ischemic heart. Wang S, Zhang Z, Lin X, Xu DS, Feng Y, Ding K.Glycobiology. 2010 Jan;20(4):473-84. Epub 2009 Dec 15. 7. G{alpha}q-mediated plasma membrane translocation of sphingosine kinase-1 and cross-activation of S1P receptors. Ter Braak M, Danneberg K, Lichte K, Liphardt K, Ktistakis NT, Pitson SM, Hla T, Jakobs KH, Heringdorf DM.Biochim Biophys Acta. 2009 Feb 2. [Epub ahead of print] 8. Sphingosine kinase 1 is essential for proteinase-activated receptor-1 signalling in epithelial and endothelial cells. Billich A, Urtz N, Reuschel R, Baumruker T.Int J Biochem Cell Biol. 2009 Jul;41(7):1547-55. Epub 2009 Jan 8.

Additional Info

Recognizes human SPHK1.

Alternative Names

EC=2.7.1.91