Spinesin (TMPRSS5, Transmembrane Protease Serine 5, Spinesin)

Spinesin, also known as Transmembrane Protease, Serine 5 (TMPRSS5) is a type II transmembrane protein that includes a short N-terminal cytoplasmic tail (aa 1-49), a transmembrane domain (aa 50-70), a stem region and a serine protease domain (residues 71-457). Spinesin occurs predominately in neurons, in their axons, at the synapsis of motor neurons in the spinal cord, and in some oligodendrocytes. The amino acid sequence of human Spinesin is 94%, 82%, 81% and 79% identical to that of chimpanzee, canine, rat and mouse.\n\nHybridoma: \nProduced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, NS0-derived, recombinant human Spinesin (rhSpinesin; aa 71-457, Accession # NP_110397).\n\nApplications: \nImmunoprecipitation: This antibody was used at a concentration of 25ug/ml to immunoprecipitate rhSpinesin from conditioned cell culture medium. The recovered rhSpinesin can be detected in Western blots using a polyclonal antibody. \nWestern Blot: This antibody can be used at 1-2ug/ml with the appropriate secondary reagents to detect human Spinesin. Using a colorimetric detection system, the detection limit for rhSpinesin is approximately 25 ng/lane under non-reducing conditions. Use of this antibody under reducing conditions is not recommended. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. For Western blot under reducing conditions, AF2495 and BAF2495 are recommended. \nDirect ELISA: This antibody can be used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect human Spinesin. The detection limit for rhSpinesin is approximately 20 ng/well. \nOptimal dilutions should be determined by each laboratory for each application. \n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S5510-10

Size

100ug

Applications

ELISA, IP, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS, 40-50% glycerol. If 0.2ml is used, the concentration will be 0.5mg/ml.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

Additional Info

Selected for its ability to detect human Spinesin in direct ELISAs and Western Blots.