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sPLA2 Assay Kit

Cat no: 765001

sPLA2 Assay Kit

Cayman's sPLA2 Assay provides an accurate and convenient method for measurement of sPLA2 activity. This assay uses the 1,2-dithio analog of diheptanoyl phosphatidylcholine which serves as a substrate for most PLA2s with the exception of cPLA2. Upon hydrolysis of the thioester bond at the sn-2 position by PLA2, free thiols are detected using DTNB.

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SPECIFICATIONS

Catalog Number

765001

Form

96 Well

P Type

Assay Kits|Phospholipase|Phospholipids|Eicosanoids

Weight

0

Storage Temp

-20

Shipping Temp

-20

Additional Info

Phospholipase A2 (PLA2) catalyzes the hydrolysis of phospholipids at the sn-2 position yielding a free fatty acid and a lysophospholipid. The release of arachidonic acid from membrane phospholipids by PLA2 is believed to be a key step in the control of eicosanoid production within the cell. The Cayman Chemical secretory PLA2 (sPLA2) Assay kit provides an accurate and convenient method for measurement of sPLA2 activity. This assay uses the 1,2-dithio analog of diheptanoyl phosphatidylcholine which serves as a substrate for most PLA2s (for example, bee and cobra venoms, pancreatic, etc.) with the exception of cytosolic PLA2. Upon hydrolysis of the thio ester bond at the sn-2 position by PLA2, free thiols are detected using DTNB (5,5-dithio-bis-(2-nitrobenzoic acid)).

SUPPLIER INFO

Cayman Chemical Company

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