

Supplier:
Cayman Chemical CompanyCat no: 765001
sPLA2 Assay Kit
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SPECIFICATIONS
Catalog Number
765001
Form
96 Well
P Type
Assay Kits|Phospholipase|Phospholipids|Eicosanoids
Weight
0
Storage Temp
-20
Shipping Temp
-20
Additional Info
Phospholipase A2 (PLA2) catalyzes the hydrolysis of phospholipids at the sn-2 position yielding a free fatty acid and a lysophospholipid. The release of arachidonic acid from membrane phospholipids by PLA2 is believed to be a key step in the control of eicosanoid production within the cell. The Cayman Chemical secretory PLA2 (sPLA2) Assay kit provides an accurate and convenient method for measurement of sPLA2 activity. This assay uses the 1,2-dithio analog of diheptanoyl phosphatidylcholine which serves as a substrate for most PLA2s (for example, bee and cobra venoms, pancreatic, etc.) with the exception of cytosolic PLA2. Upon hydrolysis of the thio ester bond at the sn-2 position by PLA2, free thiols are detected using DTNB (5,5-dithio-bis-(2-nitrobenzoic acid)).
Applications
ELISA, IF, IP, WB
Hosts
Rabbit
Applications
ELISA, IP, WB
Hosts
Rabbit
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