Splicing Factor 2 (SF2, ASF)

Numerous protein and ribonucleoprotein components are required to catalyze pre-mRNA splicing. The SR proteins are essential splicing factors. They regulate alternative splicing of many pre-mRNAs. Alternative splicing is a major mechanism for controlling expression of cellular and viral genes. SR proteins are a family of highly conserved serine/arginine rich RNA-binding proteins. Nine human SR proteins including SF2/ASF are currently known. Based on primary sequence and on motifs shared with other proteins, human SF2/ASF is a 248aa protein composed of four domains. SF2/ASF exists predominantly or exclusively in a highly phosphorylated state in a majority of cells.\n\nApplications: \nSuitable for use in Immunofluorescence, Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 0.5-1ug/ml\nOptimal dilutions to be determined by the researcher.\n\nMolecular Weight: \nCalculated MW: 27.7kD\nWestern Blot: ~33kD (Doublet or triplet bands sometimes evident due to phosphorylation differences). \n\nRecommended Control:\nHeLa cells\n\nSource: Ascites\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

S5555-50

Size

100ug

Applications

IF, IP, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG2b

References

1. Hanamura, A., et al., RNA 4: 430-444 (1998). 2. Caceres, J.F., et al., J. Cell. Biol. 138(2): 225-238 (1997). 3. Liu, H.X., et al., Genes Dev. 12(13): 1998-2012 (1998).

Additional Info

Recognizes the N-terminal portion (aa1-97) of human SF2/ASF protein comprising the RNA-recognition motif (RRM1). Reactivity is not affected by phosphorylation state of SF2/ASF. Species Crossreactivity: mouse and rat. Crossreactivity expected with other species because SF2/ASF is highly conserved.