Splicing Factor 3B Subunit 4 (SF3B4, SF3b50, Pre-mRNA Splicing Factor SF3b 49kD Subunit, Spliceosome-associated Protein 49, SAP 49, SAP49)

Splicing factor SF3B4, also known as SAP49, is a ubiquitously expressed splicing factor found in the nuclei of eukaryotic cells, although it migrates into the cytoplasm of dividing cells. It was originally identified by as the protein most efficiently UV cross-linked to the A, B and C spliceosomal complexes (1). The protein contains two of the highly conserved RRM type RNA recognition motifs, each corresponding to a small ~70 amino acid structure, consisting of 4 beta strands and two alpha-helices. Proteins containing these proteins are believed to have a role in the regulation of mRNA splicing. The protein runs on SDS-PAGE gels at an apparent molecular weight of 49kD. This protein is known as splicing factor 3b, subunit 4, 49kD SAP49, spliceosome-associated protein (U2 snRNP), Hsh49 and MGC108282. Antibodies to this protein are good markers of nuclei. The HGNC name for this protein is SF3B4.\n\nApplications:\nSuitable for use in Immunofluorescence and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nImmunofluorescence: 1:1000\nWestern Blot: 1:1000 using chemiluminescence\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

031429

Size

100ul

Applications

IF, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Bov, Prc

Form

Supplied as a liquid in PBS, 10mM sodium azide.

P Type

Mab

Purity

Purified by affinity chromatography.

Isotype

IgG2b,k

References

1. Champion-Arnaud P, Reed R. The prespliceosome components SAP 49 and SAP 145 interact in a complex implicated in tethering U2 snRNP to the branch site. Genes Dev. 8:1974-83 (1994).

Additional Info

Recognizes SF3B4 from human, bovine, porcine, mouse, rat and other mammals. Since the SF3B4 protein sequence is highly conserved across species barriers, it is likely that the antibody is effective on many other species also.

Alternative Names

Splicing Factor 3b Subunit 4 49 kD, Spliceosome-associated Protein (U2 snRNP)