Splicing Factor 3b Subunit 4 (Splicing Factor 3b Subunit 4 49kDa, SF3B4, SF3b49, SF3b50, MGC10828, Pre-mRNA Splicing Factor SF3b 49kD Subunit, Spliceosome Associated Protein (U2 snRNP), Spliceosome-associated Protein (U2 snRNP), Spliceosome-associated Pro

The SF3B4 gene encodes one of four subunits of the splicing factor 3B. The protein encoded by this gene cross-links to a region in the pre-mRNA immediately upstream of the branchpoint sequence in pre-mRNA in the prespliceosomal complex A. It also may be involved in the assembly of the B, C and E spliceosomal complexes. In addition to RNA-binding activity, this protein interacts directly and highly specifically with subunit 2 of the splicing factor 3B. This protein contains two N-terminal RNA-recognition motifs (RRMs), consistent with the observation that it binds directly to pre-mRNA.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nCellular Localization: Nuclear\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S5555-50G

Size

100ug

Applications

ELISA, WB

Hosts

Goat

Reactivities

Hum, Mouse, Dro/Arth, Xen/Amph, ZF/Fish

Form

Supplied as a liquid in TBS, pH 7.2, 0.5% BSA.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

Champion-Arnaud P & Reed R The prespliceosome components SAP 49 and SAP 145 interact in a complex implicated in tethering U2 snRNP to the branch site. Genes Dev 8:1974-83 (1994). <PUBMED:7958871>

Additional Info

Species Crossreactivity: Crossreacts with Human. Expected to crossreact with Mouse (100% identity with immunogen), Xenopus laevis (100% identity with immunogen), Zebrafish (100% identity with immunogen) and Drosophilia melanogaster (100% identity with immunogen) due to sequence homology.