Splicing Factor, Arginine/Serine-rich 2 (SFRS2, PR264, Protein PR264, SFRS2A, Splicing Component 35kD, SC 35, SC35, SC-35, Splicing Factor SC35, Splicing Speckle, Splicing Speckles, SRp30b)

SC35 is involved in pre-mRNA splicing and is found in the bodies in the nucleus referred to as speckles, SC 35 domains or splicing factor compartments (SFCs). The SC-35 clone is frequently used as a marker for these bodies, the role of which is controversial. Speckles correspond largely if not entirely to ultrastructures termed interchromatin granule clusters (IGCs). SC35 is excluded from the nucleolus.\n\nApplications: \nSuitable for use in Immunofluorescence, ELISA, Western Blot, Immunoprecipitation, and Immunohistochemistry (paraffin-embedded sections). Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nCellular Localization: Nuclear\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S5554-52A

Size

100ul

Applications

ELISA, IF, IHC, IP, WB

Hosts

Mouse

Reactivities

Hum, Rat, Dro/Arth, Xen/Amph

Form

Supplied as a liquid, 0.09% sodium azide.

P Type

Mab

Purity

Ascites

Isotype

IgG1

References

1. Fu XD et al. General splicing factors SF2 and SC35 have equivalent activities in vitro, and both affect alternative 5' and 3' splice site selection. Proc Natl Acad Sci U S A 89:11224-8 (1992). <PUBMED:1454802>

Additional Info

Recognizes a phospho-epitope on the non-snRNP (small nuclear ribonucleoprotein particles) factor SC-35. Reacts with the splicing factor SC-35 and with the SC-35-related non-snRNP factor SF2/ASF. Species Crossreactivity: Crossreacts with Human, Rat, Xenopus laevis, Drosophilia melanogaster and Newt.