SPNXB, ID (SPANXB1, SPANXB, Sperm protein associated with the nucleus on the X chromosome B/F, Cancer/testis antigen 11.2, Nuclear-associated protein SPAN-Xb, Nuclear-associated protein SPAN-Xf, SPANX family member B/F) (Biotin)

Temporally regulated transcription and translation of several testis-specific genes is required to initiate the series of molecular and morphological changes in the male germ cell lineage necessary for the formation of mature spermatozoa. This gene is a member of the SPANX family of cancer/testis-associated genes, which are located in a cluster on chromosome X. The SPANX genes encode differentially expressed testis-specific proteins that localize to various subcellular compartments. This particular gene maps to chromosome X in a head-to-tail orientation with SPANX family member B1 and appears to be a duplication of that locus. The SPANXB genes are unique members of this gene family, since they contain an additional 18 nt in their coding region compared to the majority of family members. Although the protein encoded by this gene contains consensus nuclear localization signals, the major site for subcellular localization of expressed protein is in the cytoplasmic droplets of ejaculated spermatozoa. This protein provides a biochemical marker for studying the unique structures in spermatazoa, while attempting to further define its role in spermatogenesis.\n\nApplications:\nSuitable for use in Western Blot, ELISA\n\nRecommended Dilution:\nELISA: 1:1,000\nWestern Blot: 1:100-500\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

042247-Biotin

Size

200ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

Conjugates

Biotin

Form

Supplied as a liquid in PBS, pH 7.2. Contains ~0.09% sodium azide. Conjugated to Biotin.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

Hansen, S., et al. Syst Biol Reprod Med 55, 18-26 (2010) :\nHansen, M.A., et al. Mol. Reprod. Dev. 75(2):219-229(2008)\nKouprina, N., et al. Genome Res. 15(11):1477-1486(2005)\nRoss, M.T., et al. Nature 434(7031):325-337(2005)\nZendman, A.J., et al. Gene 309(2):125-133(2003)

Additional Info

Human

Alternative Names

SPANXB1, SPANXB, Sperm protein associated with the nucleus on the X chromosome B/F, Cancer/testis antigen 11.2, Nuclear-associated protein SPAN-Xb, Nuclear-associated protein SPAN-Xf, SPANX family member B/F