SPOCK2 (Testican 2, KIAA0275) (Biotin)

Testican 2 (also SPOCK2) is a 60-140kD, secreted multidomain proteoglycan that belongs to the BM-40/SPARC family of extracellular proteins. It is expressed by neurons (both excitatory and GABAergic) plus brain endothelial cells, and appears to abrogate testican 1 and 3 mediated inhibition of MT1- and MT3-MMP activity. Mature human Testican 2 is 402 amino acids (aa) in length (aa23-424). It contains a unique 66aa N terminus, followed by a Kazal-like follistatin domain (aa89-192), a collagen-binding domain (aa210-219), a Ca++ binding region, one thyroglobulin-like segment (aa310-376) and an acidic C-terminus that contains two potential GAG attachment sites at Ser383 and Ser388. The wide variation in native MW reflects the capabilities of differing cells to add glycan adducts to the molecule. Typically, it appears to lie belween 80-125kD. There are potential splice variants. One shows an alternative start site at Met110, a second shows a deletion of aa64-66, while a third contains a 14aa substitution for aa64-242. Mature human Testican 2 is 94% aa identical to mouse and rat Testican 2.\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.1ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute in sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

145613

Size

50ug

Applications

WB

Hosts

Goat

Reactivities

Hum

Conjugates

Biotin

Form

Supplied as a lyophilized powder in PBS, BSA. Labeled with Biotin. Reconstitute in Tris-buffer saline, pH 7.3.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

Additional Info

Recognizes human Testican 2/SPOCK2 in Western blots. Species Crossreactivity: In Western blots, <5% with recombinant human Testican 1 and rhTestican 3.