Home  >  Products  >  Sprouty 1 (hSPRY1, Sprouty Homolog 1 Antagonist of FGF Signaling, Protein Sprouty Homolog 1, Sprouty Homolog 1, SPRY1, Spry-1)
Sprouty 1 (hSPRY1, Sprouty Homolog 1 Antagonist of FGF Signaling, Protein Sprouty Homolog 1, Sprouty Homolog 1, SPRY1, Spry-1)

Sprouty 1 (hSPRY1, Sprouty Homolog 1 Antagonist of FGF Signaling, Protein Sprouty Homolog 1, Sprouty Homolog 1, SPRY1, Spry-1)

Cat no: S5620-01G

Supplier: United States Biological
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SPRY1 is a 34-38kD member of the sprouty family of proteins. It is widely expressed, being found in multiple embryonic and adult tissues. SPRY1 is considered a negative regulator of cellular signaling. In particular, it appears to both inhibit MAP kinase signaling following RTK activation, and block TCR signaling following antigen activation. It interacts with a number of molecules, including PLC-g 1, LAT, CBL, caveolin-1 and SPRY2. Human SPRY1 is 319aa in length. It contains one CBL-TKB binding site aa51-57 that is phosphorylated at Tyr53, a Ser-rich region aa112-131, and a Cys-rich domain aa181-306 that mediates intracellular translocation. SPRY1 undergoes serine phosphorylation, ubiquitination and palmitoylation, the latter which induces SPRY1 to associate with cell membranes. Over aa1-178, human SPRY1 shares 76% aa identity with mouse SPRY1. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:16,000 Western Blot: 1-3ug/ml Immunohistochemistry (Paraffin): 1-3ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: S5620-01G
Reactivities: Human, Mouse
Hosts: Goat
Applications: ELISA, Immunohistochemistry, Western Blot
Size: 100ug
Form: Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
P type: Pab
Purity: Purified by immunoaffinity chromatography.
References: 1. Schaaf G, Hamdi M, Zwijnenburg D, Lakeman A, Geerts D, Versteeg R, Kool M. Silencing of SPRY1 triggers complete regression of rhabdomyosarcoma tumors carrying a mutated RAS gene. Cancer Res. 2010 Jan 15;70(2):762-71. Epub 2010 Jan 12.
Additional info: Recognizes human SPRY1. Species Crossreactivity: Mouse.

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