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Sprouty-related EVH1 domain-containing Protein 1, IN2 (Spred1, NFLS, FLJ33903)

Sprouty-related EVH1 domain-containing Protein 1, IN2 (Spred1, NFLS, FLJ33903)

Cat no: S5620-35B

Supplier: United States Biological
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The Ras-MAP kinase pathway is essential for the differentiation of neuronal cells and myocytes; it is inhibited by Spred1, a member of the Sprouty family of proteins. Spred1 acts by suppressing the phosphorylation and activation of Raf. The Spred proteins have also been implicated in the negative feedback regulation of FGF signaling in embryogenesis and angiogenesis. Further studies have shown that expression levels of Spred1 and Spred2 proteins are inversely correlated with the incidence of tumor invasion and metastasis in human hepatocellular carcinoma (HHC), suggesting that these proteins could be useful as prognostic factors and therapeutic targets in HCC. Defects in this gene are a cause of neurofibromatosis type 1-like syndrome (NFLS). This Spred1 antibody is predicted to have no cross-reactivity to Spred2 or Spred3. Applications: Suitable for use in Elisa and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1-2ug/ml Optimal dilutions to be determined by the researcher. Positive Control: Human Brain Tissue Lysate Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: S5620-35B
Reactivities: Human, Mouse, Rat
Hosts: Rabbit
Applications: ELISA, Western Blot
Size: 100ug
Form: Supplied as a liquid in PBS, 0.02% sodium azide.
P type: Pab
Purity: Purified by immunoaffinity chromatography.
References: 1. Wakioka T, Sasaki A, Kato R, et al. Spred is a Sprouty-related suppressor of Ras signalling. Nature 2001; 412:647-51. 2. Minowada G, Jarvis LA, Chi CL, et al. Vertebrate Sprouty genes are induced by FGF signaling and can cause chondrodysplasia when overexpressed. Development 1999; 126:4465-75. 3. Lee SH, Schloss DJ, Jarvis L, et al. Inhibition of angiogenesis by a mouse sprouty protein. J. Biol. Chem. 2001; 276:4128-33. 4. Yoshida T, Hisamoto T, Akiba J, et al. Spreds, inhibitors of the Ras/ERK signal transduction, are dysregulated in human hepatocellular carcinoma and linked to the malignant phenotype of tumors. Oncogene 2006; 25:6056-66.
Additional info: Recognizes human Spred1. Species Crossreactivity: Mouse and rat.

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