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SPSB1, Recombinant, Human, aa24-233, His-tag (SSB-1, SSB1)

Cat no: S5999-65

SPSB1, Recombinant, Human, aa24-233, His-tag (SSB-1, SSB1)

SPRY domain-containing SOCS box protein 1 (SPSB1), also known as SSB1, is a member of the SOCS box protein subfamily. This protein contains a central SPRY domain and a C-terminal SOCS box. Although some of the SOCS protein subfamilies function as adaptors for a large family of ubiquitin-protein isopeptide ligases to regulate certain signaling pathways, the function of the SSB subfamily remains to be determined. SPSB1 may play an important role in enhancing the HGF-induced Erk-Elk-1-SRE pathway. Over expression of SPSB1 exhibited no effect on the basal level or epidermal growth factor-induced SRE-luciferase activity.\n\nSource: \nRecombinant corresponding to aa24-233 from human SPSB1, fused to His-tag at N-terminus expressed in E.coli.\n\nAA Sequence:\nMGSSHHHHHH SSGLVPRGSH MQELQGLDYC KPTRLDLLLD MPPVSYDVQL LHSWNNNDRS LNVFVKEDDK LIFHRHPVAQ STDAIRGKVG YTRGLHVWQI TWAMRQRGTH AVVGVATADA PLHSVGYTTL VGNNHESWGW DLGRNRLYHD GKNQPSKTYP AFLEPDETFI VPDSFLVALD MDDGTLSFIV DGQYMGVAFR GLKGKKLYPV VSAVWGHCEI RMRYLNGLDP E\n\nMolecular Weight: \n~26.1kD (231aa) confirmed by MALDI-TOF\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S5999-65

Size

250ug

Form

Supplied as a liquid in 20mM Tris-HCl buffer, pH8.0, 1mM DTT, 0.1M sodium chloride, 30% glycerol.

Purity

~90% (SDS-PAGE)

References

1. Woo J S., et al. (2006) Equb. 25(6):1353-63. 2. Wang D., et al. (2005) J Biol Chem. 280(16):16393-401.

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