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Products SREBP-1 Transcription Factor Assay Kit
| Catalogue number: | 10010854 |
| Weight: | 0 |
| Form: | 96 Well |
| P type: | Assay Kits|Intracellular Signaling |
| Shipping temp: | -80 |
| Storage temp: | -80 |
| Additional info: | Three known isoforms of SREBP (sterol regulatory element binding protein) transcription factors have been characterized: SREBP-1a, SREBP-1c, and SREBP-2. SREBP-1c acts primarily to activate genes required for fatty acid synthesis, such as acetyl CoA carboxylase, fatty acid synthase, and long chain fatty acid elongase. In addition, SREBP-1c may also contribute to the regulation of glucose uptake and synthesis through induction of glucokinase. SREBP-1c has important clinical implications in the treatment of many diseases including obesity, diabetes mellitus, insulin resistance, and non-alcoholic fatty liver disease (NAFLD). Cayman's SREBP-1 Transcription Factor Assay is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity. SREBP-1 contained in nuclear extracts and whole cell lysates binds to a dsDNA SREBP response element immobilized to the wells of a 96-well plate. SREBP-1 is detected by addition of a specific primary antibody directed against SREBP-1. A secondary antibody conjugated to HRP is used to provide a sensitive colorimetric readout at 450 nm. |
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