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SSB (Sjogren Syndrome Antigen B, La autoantigen, Lupus La Protein)

Cat no: S1014-09F5

SSB (Sjogren Syndrome Antigen B, La autoantigen, Lupus La Protein)

SSB is a highly abundant, largely nuclear phosphoprotein that binds to newly synthesized transcripts of RNA polymerase III. The RNAs bound by this protein include precursors to 5S rRNA, tRNA, the spliceosomal U6 RNA, the signal recognition particle SRP RNA, and the cytoplasmic Y RNAs. At least part of the binding site for SSB on these RNAs is the sequence UUU(OH), which is at the 3-prime end of most newly synthesized transcripts of RNA polymerase III (1). SSB was originally defined by its reactivity with autoantibodies from patients with Sjogren syndrome and systemic lupus erythematosus (2).\n\nApplications:\nSuitable for use in Western Blot, Immunoprecipitation, Immunocytochemistry and Immunohistochemistry. Other applications have not been tested. \n\nRecommended Dilutions: \nWestern Blot: 1:10,000-1:50,000\nImmunohistochemistry: 1:250-1:500\nImmunoprecipitation: 1:10-1:100\nImmunocytochemistry: 1:50-1:100\nOptimal dilutions to be determined by the researcher. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.\n\nManufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.

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SPECIFICATIONS

Catalog Number

S1014-09F5

Size

100ul

Applications

ICC, IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, pH 7.2, 0.05% BSA, 0.01% sodium azide, 50% glycerol.

P Type

Mab

Purity

Supernatant

References

1. Van Horn DJ, et al. RNA 3(12):1434-43, 1997 2. Entrez Gene: gene-centered information at NCBI. Nucleic Acids Res. 2005 Jan 1; 33: D54-8.

Additional Info

Recognizes human SSB. Species crossreactivity: Mouse, rat.

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