ST6GALNAC2, Recombinant, Mouse (6468-GT-020Alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase 2, Gal-beta-1,3-GalNAc alpha-2,6-sialyltransferase, GalNAc alpha-2,6-sialyltransferase II, ST6GalNAc II, ST6GalNAcII, Sialyltransferase 7B, SIAT7-B, Siat7

Sialic acid molecules attached to glycoproteins or glycosphingolipids play important roles in various biological processes such as immune recognition, pathogen infection, and cell adhesion. Sialyltransferases are key enzymes that regulate the serum levels of sialic acid-containing molecules. Alpha-N-acetylgalactosaminide a-2,6-sialyltransferase 2, encoded by the ST6GALNAC2 gene, is a type II membrane protein localized in the Golgi network and catalyzes 2,6-sialylation of the Tn antigen (GalNAc-O-Ser/Thr) (1). Sialyl-Tn antigen is highly expressed in several human carcinomas and is associated with carcinoma aggressiveness and poor prognosis. Cells transfected with ST6GALNAC2 synthesized the sialyl-6T structure [GalB1-3 (Neu5Aca2-6) GalNAc-O-Ser/Thr] (2). In vitro analysis revealed that the enzyme can use both GalB1-3GalNAc and Neu5Aca2-3GalB1-3GalNAc as acceptor substrates (3). Recently, it has been reported that disregulation of this gene is involved in the pathogenesis of IgA nephropathy, a common kidney disease that occurs when antibody IgA accumulates in kidneys (4). The enzyme may also be used to synthesize a cancer vaccine (5, 6). At the protein level, mouse ST6GALNAC2 shows 75 sequence identity with the human homologue. The activity of this enzyme has been measured using a phosphatase-coupled assay (7).\n\nSource: \nRecombinant corresponding to aa28-373 from mouse ST6GALNAC2, fused with 6-His tag at C-terminal and human CD33 signal sequence at N-terminal, expressed in myeloma cell line, NSO cells.\n\nMolecular Weight: \n~40kD\n\nEndotoxin Level:\n(same/less than)1EU/1ug (LAL)\n\nBiological Activity:\nMeasured by its ability to transfer Neu5Ac from CMP-Neu5Ac to bovine asialofetuin. \n\nSpecific Activity: \n>225pmoles/min/ug, as measured under the described conditions.\n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing and store at -70 degrees C. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

S0550-04

Size

20ug

Form

Supplied as a liquid in Tris, sodium chloride.

Purity

~95% (SDS-PAGE)

References

1. Kono, M., et al. (2000) Biochem. Biophys. Res. Commun. 272:94. 2. Marcos, N.T., et al. (2004) Cancer Res. 64:7050. 3. Samyn-Petit, B., et al. (2000) Biochim. Biophys. Acta 1474:201. 4. Li, G.S., et al. (2007) Hum. Mutat. 28:950. 5. Julien, S., et al. (2009) Br. J. Cancer 100:1746. 6. Sewell, R., et al. (2006) J. Biol. Chem. 281:3586. 7. Wu, Z.L., et al. (2010) Glycobiology doi:10.1093/glycob/cwq187.