Gangliosides are acidic glycosphingolipids that contain one or more sialic acid residues. They are abundant in the nervous system, where they play crucial modulatory roles in cellular recognition, interaction, adhesion, and signal transduction, particularly during early developmental stages. The expression of gangliosides in the nervous system is developmentally regulated through various sialyltransferases. ST6GALNAC5 is a sialyltransferase involved in the biosynthesis of ganglioside GD1a (NeuAca2,3GalB1,3GalNAcB1, 4(NeuAcA2,3)GalB1, 4GlcB1-Cer) from GM1b (NeuAcA2,3GalB1,3GalNAcB1,4GalB1,4GlcB1-Cer), and its expression is restricted to the brain. ST6GALNAC5 has been identified as a key player in the metastasis of breast cancer cells to the brain by potentially enabling the cancer cells to cross the blood-brain barrier. The recombinant ST6GALNAC5 was active on fetuin from fetal calf serum when assayed using a phosphatase-coupled method suggesting the substrate specificity of ST6GALNAC5 may require further characterization.
Source:
Recombinant corresponding to aa30-336 from human ST6GALNAC5, fused to 6-His-tag and with an N-terminal CD33 signal peptide, expressed in Chinese Hamster Ovary cell line.
Molecular Weight:
~36kD
Biological Activity:
Measured by its ability to transfer Neu5Ac from CMP-Neu5Ac to fetuin of fetal calf serum.
Specific Activity:
>40pmol/min/ug
Endotoxin: ~1EU/1ug (LAL)
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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