Home  >  Products  >  STAP1, Recombinant, Human, aa1-295, His-Tag (Signal-transducing Adaptor Protein 1, STAP-1, BCR Downstream-signaling Protein 1, Docking Protein BRDG1, Stem Cell Adaptor Protein 1, BRDG1)
STAP1, Recombinant, Human, aa1-295, His-Tag (Signal-transducing Adaptor Protein 1, STAP-1, BCR Downstream-signaling Protein 1, Docking Protein BRDG1, Stem Cell Adaptor Protein 1, BRDG1)

STAP1, Recombinant, Human, aa1-295, His-Tag (Signal-transducing Adaptor Protein 1, STAP-1, BCR Downstream-signaling Protein 1, Docking Protein BRDG1, Stem Cell Adaptor Protein 1, BRDG1)

Cat no: 045461

Supplier: United States Biological
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STAP1, also known as signal-transducing adaptor protein 1, appears to functions as a docking protein acting downstream of Tec tyrosine kinase in B cell antigen receptor signaling. The protein is directly phosphorylated by Tec in vitro where it participates in a positive feedback loop, increasing Tec activity. Source: Recombinant corresponding to aa1-295 from human STAP1, fused to His-tag at N-terminus, expressed in E.coli. Molecular Weight: ~36.8kD (319aa) confirmed by MALDI-TOF AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMMAKKP PKPAPRRIFQ ERLKITALPL YFEGFLLIKR SGYREYEHYW TELRGTTLFF YTDKKSIIYV DKLDIVDLTC LTEQNSTEKN CAKFTLVLPK EEVQLKTENT ESGEEWRGFI LTVTELSVPQ NVSLLPGQVI KLHEVLEREK KRRIETEQST SVEKEKEPTE DYVDVLNPMP ACFYTVSRKE ATEMLQKNPS LGNMILRPGS DSRNYSITIR QEIDIPRIKH YKVMSVGQNY TIELEKPVTL PNLFSVIDYF VKETRGNLRP FICSTDENTG QEPSMEGRSE KLKKNPHIA Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: 045461
Size: 100ug
Form: Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 1mM DTT, 0.1M sodium chloride, 30% glycerol.
Purity: ~95% (SDS-PAGE)
References: 1. Ohya K., et al. (1999) Proc. Natl. Acad. Sci. USA 96:11976-11981. 2. Beausoleil S.A., et al. (2006) Nat. Biotechnol. 24:1285-1292.

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