STAT 1, Recombinant, Human (Signal Transducer and Activator of Transcription 1)

Recombinant Human STAT1 produced in E.Coli is a non-glycosylated polypeptide chain having a molecular mass of 90kD. Recombinant STAT1 is purified by proprietary chromatographic techniques. This protein is the full-length form of the protein with An amino-terminal His-tag.\n\nUnit Definition: \nRecombinant human STAT1 is phosphorylatable in vitro, using either immunoprecipitated JAK1 or JAK2, or whole cell extracts derived from interferon- -stimulated HEK 293 cells. This phosphorylation can be monitored by Western blot analysis using phosphorylation site specific antibody directed to STAT [pY701], in conjunction with chemiluminescence detection methods. Optimization of the cell stimulation protocol, cell lysis procedure, and reaction conditions may be required for each specific application.\n\nStorage: \nStore at 4 degrees C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degrees C for longer periods of time. Please avoid freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

S7969-03H

Size

2ug

Form

Supplied as a lyophilized powder without additives.

Purity

(same/more than) 95% (SDS-PAGE)

References

1. [STAT1 antisense oligonucleotides inhibit secretion of TNF-alpha, IL-8 and NO in alveolar macrophages of rats suffering from interstitial pulmonary fibrosis.]\n\nXi Bao Yu Fen Zi Mian Yi Xue Za Zhi 2006 Jul;22(4):487-9\n\n2. Serine-phosphorylated STAT1 is a prosurvival factor in Wilms' tumor pathogenesis.\n\nOncogene 2006 Jun 26;\n\n3. Cutting Edge: STAT1 and T-bet Play Distinct Roles in Determining Outcome of Visceral Leishmaniasis Caused by Leishmania donovani.\n\nJ Immunol 2006 Jul 1;177(1):22-5\n\n4. Regulation of Stat1 protein expression by phenylalanine 172 in the coiled-coil domain.\n\nBiochem Biophys Res Commun 2006 Aug 4;346(3):1062-6\n\n5. Transcriptional activation of c-Fos by constitutively active Galpha(16)QL through a STAT1-dependent pathway.\n\nCell Signal 2006 May 9;\n\n6. The downstream modulator of interferon-gamma, STAT1 is not genetically associated to the Dutch coeliac disease population.\n\nEur J Hum Genet 2006 Jun 14;