STAT 5, phosphorylated (Tyr694) Kit, BioAssay(TM) (Signal Transducer and Activator of Transcription 5)

The Phosphorylated Stat5 (Tyr694) Antibody Kit provides reagents and protocols for the rapid analysis of the phosphorylation status of Stat5 at tyrosine 694. \n\nStat5 is activated in response to a wide variety of ligands including IL-2, GM-CSF, growth hormone and prolactin. Phosphorylation on residue Tyr694 is obligatory for Stat5 activation. Stat5 has been found to be constitutively active in some leukemic cell types. Phosphorylated St at5 is found in some endothelial cells treated with IL-3, which suggests its involvement in angiogenesis and cell motility. Stat5a and-b are independently regulated and activated in various cell types. For instance, both isoforms are activated in response to IFN-a in B cells, but only Stat5a is phosphorylated in response to IFN-a in HeLa cells.\n\nKit Components:\nS7969-95A: Stat 5 phosphorylated (Tyr694),1x100ul Purified by immunoaffinity chromatography.\nSupplied as a liquid in 10mM sodium HEPES, pH 7.5, 0.1mg/ml BSA and 50% glycerol. \nS7969-95B: Stat 5, 1x100ul. Purified by immunoaffinity chromatography.\nSupplied as a liquid in 10mM sodium HEPES, pH 7.5, 0.1mg/ml BSA and 50% glycerol. \nS7969-95C: Stat 5, Total HeLa Cell Extract, Negative Control, 1x40ul Supplied as a liquid in 62.5mM Tris-HCl, pH 6.8, 2% SDS, 50mM DTT, 0.01% bromophenol blue, 10% glycerol.\nS7969-95D: Stat 5, Total HeLa Cell Extract, Positive Control, Treated with IFN-a. 1x40ul. Supplied as a liquid in 62.5mM Tris-HCl, pH 6.8, 2% SDS, 50mM DTT, 0.01% bromophenol blue, 10% glycerol.\nW1020: Western Blot Detection System, IgG, HRP, Chemiluminescent, BioAssay(TM), Rabbit\nW1020A: Western Blot Detection System, IgG (HRP), 1x50ul\nW1020B: Western Blot Detection System, Biotin (HRP), 1x100ul\nW1020C: Western Blot Detection System, Protein MW Marker (Biotin), 1x100ul\nW1020D: Western Blot Detection System, LumiGLO(R) Reagent (20X), 1x5ml\nW1020E: Western Blot Detection System, Peroxide Reagent (20X), 1x5ml\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

S7969-95

Size

1Kit

References

1 Gouilleux, F. et al. (1994) EMBO J. 13, 4361-4369. 2. Wakao, H. et al. (1994) EMBO J. 13, 2182-2191. 3. Demoulin, J.B. et al. (1999) J. Biol. Chem. 274, 25855-25861. 4. Dentelli, P. et al. (1999) J. Immunol. 163, 2151-2159. 5. Meinke, A. et al. (1996) Mol. Cell. Biol. 16, 6937-6944.