STAT1, alpha, CT (Signal Transducer And Activator Of Transcription 1-alpha/beta, Transcription Factor ISGF-3 Components p91/p84, Signal Transducer And Activator Of Transcription 1, 91kD)

STAT1alpha (Signal transducer and activator of transcription 1 alpha) is a 91kD protein belonging to the transcription factor STAT family. Members of the STAT family respond to cytokines and growth factors and are phosphorylated by receptor associated kinase to form homo- or hetro-dimers. These then translocate to the cell nucleus to act as transcription activators. STAT1 is activated by a number of different ligands including the IFN, gp130 and receptor tyrosine kinase families. STAT1 has two different forms, a 91kD form (STAT1alpha) and an 84kD form (STAT1beta), This antibody is specific for the 91kD form, STAT1alpha and does not react with STAT1beta.\n\nApplications:\nSuitable for use in Immunohistochemistry (frozen), Immunoprecipitation, and Western Blot. Other applications not tested. \n\nRecommended Dilution:\nImmunohistochemistry (frozen): 10ug/ml\nImmunoprecipitation: 2-4ug/ml\nWestern Blot: 1:1,000-1:2,000\nOptimal dilutions to be determined by the researcher. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

S7969-60B

Size

100ug

Applications

IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Leonard W.J., et al (1998) Jaks and STATs: biological implications. Annu Rev. Immunol. 16: 293-322. 2. Darnell J.E. (1997) STATs and gene regulation. Science 277: 1630-1635. 3. Schindler C., et al (1995) Transcriptional responses to polypeptide ligands: the JAK-STAT pathway. Annu Rev Biochem 64: 621-651. 4. Schindler C., et al (1992) Proteins of transcription factor ISGF-3: one gene encodes the 91-and 84-kDa ISGF-3 proteins that are activated by interferon alpha. PNAS 89: 7836-7839.

Additional Info

Recognizes human STAT1alpha. Does not react with STAT1beta. Species Crossreactivity: mouse, rat.