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Stem Cell Factor, Recombinant, Human, aa26-189 (Stem Cell Factor, CD117 c-kit, Steel Factor, Mast Cell Growth Factor, MGF)

Cat no: S7973-01H

Stem Cell Factor, Recombinant, Human, aa26-189 (Stem Cell Factor, CD117 c-kit, Steel Factor, Mast Cell Growth Factor, MGF)

Stem Cell Factor (SCF) is a glycoprotein that plays a key role in hematopoiesis acting both as a positive and negative regulator, often in synergy with other cytokines. SCF binds to and activates the SCF receptor (SCFR), a receptor tyrosine kinase. SCF stimulates the proliferation of mast cells and is able to augment the proliferation of both myeloid and lymphoid hematopoietic progenitors in bone marrow culture. It also mediates cell-cell adhesion and acts synergistically with other cytokine. Recombinant human SCF was expressed in E. coli and purified by conventional column chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.\n\nSource:\nRecombinant corresponding to aa26-189 of human Stem Cell Factor expressed in E.coli.\n\nAA Sequence: \nMEGICRNRVT NNVKDVTKLV ANLPKDYMIT LKYVPGMDVL PSHCWISEMV VQLSDSLTDL LDKFSNISEG LSNYSIIDKL VNIVDDLVEC VKENSSKDLK KSFKSPEPRL FTPEEFFRIF NRSIDAFKDF VVASETSDCV VSSTLSPEKD SRVSVTKPFM LPPVA\n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S7973-01H

Size

100ug

Form

Supplied as a liquid in PBS, pH 7.4.

Purity

(same/more than) 95% by SDS-PAGE. Purified by using conventional chromatography techniques.

References

1. Zhang, Z., et al., (2000) Proc. Natl. Acad. Sci. U.S.A. 97: 7732 (2000). 2. Okada, S., et al., Nippon Rinsho 50: 1872 (1992).

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