Stem Cell Factor, Recombinant, Human (SCF, CD117 c-kit, Steel Factor, Mast Cell Growth Factor, MGF)

Recombinant Human SCF is a single, non-glycosylated polypeptide chain containing 165 amino acids and having a molecular mass of 18409 Dalton.\n\nSequence:\nFirst five N-terminal amino acids: Met-Glu-Gly-Ile-Cys.\n\nBiological Activity:\nFully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of Human TF-1 cells is less then 2ng/ml, corresponding to a Specific Activity of 10e5 IU/mg.\n\nProtein Content:\nProtein quantitation was carried out by two independent methods:\n1. UV spectroscopy at 280 nm using the absorbency value of 0.52 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). \n2. Analysis by RP-HPLC, using a standard solution of SCF as a Reference Standard.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C..Reconstitute with sterile ddH2O, 0.1% HSA or BSA. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S7973-02

Size

2ug

Form

Supplied as a lyophilized powder from 0.02% sodium bicarbonate. Reconstitute in sterile ddH2O, 0.1% HSA or BSA to a concentration of >0.1mg/ml, which can then be further diluted to other aqueous solutions.

Purity

(same/more than) 95% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified. Endotoxin: (same/less than)0.1ng/ug (IEU/ug).

References

1. Ex vivo manipulation of umbilical cord blood-derived hematopoietic stem/progenitor cells with recombinant human stem cell factor can up-regulate levels of homing-essential molecules to increase their transmigratory potential. Zheng Y, Watanabe N, Igura K, Takaue Y, Exp Hematol 20 03 Dec;31(12):1237-46 2. Moon TC, Lee E, Murakami M, Min HK, Mol Cells 2003 Oct 31;16(2):154-60 3. Han J, Yan X, Zhi X, Protein Expr Purif 2003 Oct;31(2):311-7 4. [Expression of recombinant human stem cell factor in insect cells] Zhang D, Qin J, Zhu J, Wei Sheng Wu Xue Bao 2000 Feb;40(1):44-9 5. [The expression study of human stem cell factor (hSCF) in E. coli] Mao H, Zhao J, Di X, Zhongguo Yi Xue Ke Xue Yuan Xue Bao 1998 Aug;20(4):279-84 6. Zhang Z, Zhang R, Schlessinger J, Proc Natl Acad Sci U S A 2000 Jul 5;97(14):7732-7.