Stem Cell Factor, Recombinant, Human (SCF, SF, FPH2, c-Kit Ligand, Kit Ligand, Kitl, KITLG, KL-1, Mast Cell Growth Factor, MGF, SHEP7)

Source:\nRecombinant corresponding to 165aa of human Stromal Cell-Derived Factor-1 alpha expressed in E. coli is a single, non-glycosylated polypeptide chain.\n\nMolecular Weight: \n~18.4kD\n\nBiological Activity:\nrHuSCF is fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of human TF-1 cellsis less then 2ng/ml, corresponding to a Specific Activity of 105IU/mg.\n\nDimers and Aggregates:\n(same/less than)1% (SDS-PAGE)\n\nProtein Content:\nProtein quantitation was carried out by two independent methods:1. UV spectroscopy at 280nm using the absorbency value of 0.52 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENEcomputer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of SCF as a Reference Standard.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O, HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S7973-30T

Size

10ug

Form

Supplied as a lyophilized powder. No preservative added. Reconstitute with sterile dH2O, 0.1% HSA or BSA to (same/more than)0.1mg/ml.

Purity

~98% (RP-HPLC, Anion-exchange FPLC, SDS-PAGE) Endotoxin: (same/less than)0.1ng/ug (IEU/ug)

References

1. Successful mobilization of peripheral blood stem cells using recombinant human stem cell factor in heavily pretreated patients who have failed a previous attempt with a granulocyte colony-stimulating factor-based regimen. Bone Marrow Transplant 2005 Sep;36(5):389-96. 2. Ancestim (recombinant human stem cell factor, SCF) in association with filgrastim does not enhance chemotherapy and/or growth factor-induced peripheral blood progenitor cell (PBPC) mobilization in patients with a prior insufficient PBPC collection. Bone Marrow Transplant 2004 Oct;34(8):683-91. 3. Ex vivo manipulation of umbilical cord blood-derived hematopoietic stem/progenitor cells with recombinant human stem cell factor can up-regulate levels of homing-essential molecules to increase their transmigratory potential. Exp Hematol 2003 Dec;31(12):1237-46. 4. Degranulation and cytokine expression in human cord blood-derived mast cells cultured in serum-free medium with recombinant human stem cell factor. Mol Cells 2003 Oct 31;16(2):154-60. 5. Expression of a novel recombinant dual human stem cell factor in insect cells. Protein Expr Purif 2003 Oct;31(2):311-7. 6. [Expression of recombinant human stem cell factor in insect cells]. Wei Sheng Wu Xue Bao 2000 Feb;40(1):44-9.

Alternative Names

DKFZp686F2250