Stem Cell Factor, Recombinant, Human (SCF, SF, FPH2, c-Kit Ligand, Kit Ligand, Kitl, KITLG, KL-1, Mast Cell Growth Factor, MGF, SHEP7)

Source:\nRecombinant corresponding to 165aa of human Stem Cell Factor expressed in E.coli is a single, non-glycosylated polypeptide chain.\n\nMolecular Weight:\n~18.4kD\n\nBiological Activity:\nThe ED50 as determined by the dose-dependent stimulation of human TF-1 cellsis less then 2ng/ml, corresponding to a Specific Activity of 105 IU/mg.\n\nDimers and Aggregates:\n(same/less than)1% (SDS-PAGE)\n\nProtein Content:\nProtein quantitation was carried out by two independent methods:1. UV spectroscopy at 280nm using the absorbency value of 0.52 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENEcomputer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of SCF as a Reference Standard.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O, HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

S7973-30U

Size

10ug

Form

Supplied as a lyophilized powder. No preservative added. Reconstitute with sterile dH2O, 0.1% HSA or BSA to (same/more than)0.1mg/ml.

Purity

~98% (RP-HPLC, Anion-exchange FPLC, SDS-PAGE) Endotoxin: (same/less than)0.1ng/ug (IEU/ug)

References

1. Successful mobilization of peripheral blood stem cells using recombinant human stem cell factor in heavily pretreated patients who have failed a previous attempt with a granulocyte colony-stimulating factor-based regimen. Bone Marrow Transplant 2005 Sep;36(5):389-96. 2. Ancestim (recombinant human stem cell factor, SCF) in association with filgrastim does not enhance chemotherapy and/or growth factor-induced peripheral blood progenitor cell (PBPC) mobilization in patients with a prior insufficient PBPC collection. Bone Marrow Transplant 2004 Oct;34(8):683-91. 3. Ex vivo manipulation of umbilical cord blood-derived hematopoietic stem/progenitor cells with recombinant human stem cell factor can up-regulate levels of homing-essential molecules to increase their transmigratory potential. Exp Hematol 2003 Dec;31(12):1237-46. 4. Degranulation and cytokine expression in human cord blood-derived mast cells cultured in serum-free medium with recombinant human stem cell factor. Mol Cells 2003 Oct 31;16(2):154-60. 5. Expression of a novel recombinant dual human stem cell factor in insect cells. Protein Expr Purif 2003 Oct;31(2):311-7. 6. [Expression of recombinant human stem cell factor in insect cells]. Wei Sheng Wu Xue Bao 2000 Feb;40(1):44-9.

Alternative Names

DKFZp686F2250