SCF is a hematopoietic growth factor that exerts its activity by signaling through the c-Kit receptor. SCF and c-Kit are essential for the survival, proliferation and differentiation of hematopoietic cells committed to the melanocyte and germ cell lineages. Human SCF manifests low activity on murine cells, while murine and rat SCF are fully active on human cells. Recombinant murine SCF is an 18.3kD polypeptide containing 164aa, which corresponds to the sequence of the secreted soluble form of SCF. Manufactured using all non-animal reagents.
Source:
Recombinant protein corresponding to mouse SCF, expressed in E.coli.
Biological Activity:
Determined by the dose-dependent stimulation of the proliferation of human TF-1 cells. The ED50 was found to be 10ng/ml.
Specific Activity:
1x10e7units/mg
Endotoxin:
<0.1ng/ug (1EU/ug)
AA Sequence:
MKEICGNPVT DNVKDITKLV ANLPNDYMIT LNYVAGMDVL PSHCWLRDMV IQLSLSLTTL LDKFSNISEG LSNYSIIDKL GKIVDDLVLC MEENAPKNIK ESPKRPETRS FTPEEFFSIF NRSIDAFKDF MVASDTSDCV LSSTLGPEKD SRVSVTKPFM LPPVA
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.