Stem Cell Factor, Recombinant, Mouse (SCF, CD117 c-kit, Steel Factor, Mast Cell Growth Factor, MGF)

Recombinant murine SCF is a single, non-glycosylated polypeptide chain containing 165 amino acids.\n\nAmino Acid Sequence:\nMKEICGNPVT DNVKDITKLV ANLPNDYMIT LNYVAGMDVL PSHCWLRDMV IQLSLSLTTL LDKFSNISEG LSNYSIIDKL GKIVDDLVLC MEENAPKNIK ESPKRPETRS FTPEEFFSIF NRSIDAFKDF MVASDTSDCV LSSTLGPEKD SRVSVTKPFM LPPVA.\n\nBiological Activity:\nRecombinant Murine SCF is fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of Human TF-1 cell line is <10ng/ml, corresponding to a Specific Activity of 1x10e5 IU/mg.\n\nProtein Content:\nProtein quantitation was carried out by two independent methods:\n1. UV spectroscopy at 280nm using the absorbency value of 0.52 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). \n2. Analysis by RP-HPLC, using a standard solution of SCF as a Reference Standard.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O, 0.1% HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S7973-12

Size

2ug

Form

Supplied as a lyophilized powder from 0.02% NaHCO3, pH 6.5. Reconstitute with sterile ddH2O, 0.1% HSA or BSA to a concentration >100ug/ml.

Purity

Chromatographically purified (> 98% by SEC-HPLC, SDS-PAGE).

References

1. Ferret-Bernard, S., et al., Immunol. Lett. 91(2-3): 221-7 (2004, Feb 15). 2. Sykes, D.B., et al., Mol. Cell. Biol. 24(3): 1256-69 (2004, Feb). 3. Hartung, G., et al., Bone Marrow Transplant 32(1): 49-56 (2003, Jul). 4. Ohmura, M., et al., Biol. Reprod. 68(6): 2304-13 (2003, Jun). 5. Simpson, K., et al., Lab. Invest. 83(2): 199-206 (2003, Feb). 6. Metcalf, D., et al., Stem Cells 20(5): 460-9 (2002).