Stem Cell Factor, Recombinant, Mouse (SCF, CD117 c-kit, Steel Factor, Mast Cell Growth Factor, MGF)

Stem Cell Factor (SCF) is a cytokine made by fibroblasts and endothelial cells. SCF binds to the receptor known as c-Kit (CD117) and is thought to play a critical role in the maintenance and surivial of hematopoietic stem cells. Human SCF shows no activity on murine cells, but murine and rat SCF are active on human cells. Recombinant murine SCF produced in E. coli is a single, non-glycosylated polypeptide chain containing 164aa and having a molecular mass of 18.3kD (P20826).\n\nBiological Activity: \nThe activity is determined by the dose-dependent proliferation of human TF-1 cells and is typically <10ng/ml.\n\nAmino Acid Sequence:\nKEICGNPVT DNVKDITKLV ANLPNDYMIT LNYVAGMDVL PSHCWLRDMV IQLSLSLTTL LDKFSNISEG LSNYSIIDKL GKIVDDLVLC MEENAPKNIK ESPKRPETRS FTPEEFFSIF NRSIDAFKDF MVASDTSDCV LSSTLGPEKD SRVSVTKPFM LPPVA \n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S7973-12A

Size

2ug

Form

Supplied as a lyophilized powder from 10mM sodium phosphate buffer, pH 6.5. Reconstitute with sterile ddH2O to 0.1mg/ml. For long term storage, add a carrier protein (0.1% HSA or BSA).

Purity

~97% as determined Reducing and non-reducing SDS-PAGE. Endotoxin: (same/less than)1EU/ug (LAL).

References

1. Horv