Stem Cell Growth Factor beta, Recombinant, Human (SCGFb)

Stem Cell Growth Factor alpha and beta are hematopoietic growth factors that exert their activity at early stages of hematopoiesis. The SCGFs are non-glycosylated species-specific cytokines that can support growth of primitive hematopoietic cells, and in combination with EPO or GM-CSF, promote proliferation or erthroid or myeloid progenitors, respectively. Recombinant human SCGFb is a 25kD polypeptide containing 227 amino acid residues.\n\nEndotoxin: \n(same/less than)0.1ng/ug (1EU/ug).\n\nBiological Activity:\nDetermined by the dose dependant stimulation of the proliferation of human TF-1 cells previously grown in media containing GM-CSF. The ED50 for this effect was observed is (same/less than)7ng/ml.\n\nAmino Acid Sequence:\nMGHGARGAER EWEGGWGGAQ EEEREREALM LKHLQEALGL PAGRGDENPA GTVEGKEDWE MEEDQGEEEE EEATPTPSSG PSPSPTPEDI VTYILGRLAG LDAGLHQLHV RLHALDTRVV ELTQGLRQLR NAAGDTRDAV QALQEAQGRA EREHGRLEGC LKGLRLGHKC FLLSRDFEAQ PSASPHPLSP DQPNGGTLEN CVAQASDDGS WWDHDCQRRL YYVCEFPF\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Add carrier protein and store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n\nCountry of Origin: USA

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SPECIFICATIONS

Catalog Number

S7973-30

Size

2ug

Form

Supplied as a lyophilized powder from PBS, 1mM DTT. Reconstitute with sterile ddH2O to 0.1-1.0mg/ml. DO NOT VORTEX. Add buffer containing carrier protein for long term storage.

Purity

(same/more than)98% by SDS-PAGE and HPLC.