STK16, also known as serine/threonine-protein kinase 16, is a membrane-associated protein kinase that phosphorylates on serine and threonine residues. STK16 may be involved in secretory vesicle trafficking or intracellular signaling. Also this protein may have a role in regulating stromal-epithelial interactions that occur during ductal morphogenesis in the mammary gland. It able to autophosphorylate on Tyr residue; it is however unclear whether it has tyrosine-protein kinase toward other proteins.
Source:
Recombinant corresponding to aa1-305 from human STK16, fused to His-tag at N-terminus, expressed in E.coli.
Molecular Weight:
~37.2kD (329aa) confirmed by MALDI-TOF
AA Sequence:
MGSSHHHHHH SSGLVPRGSH MGSHMGHALC VCSRGTVIID NKRYLFIQKL GEGGFSYVDL VEGLHDGHFY ALKRILCHEQ QDREEAQREA DMHRLFNHPN ILRLVAYCLR ERGAKHEAWL LLPFFKRGTL WNEIERLKDK GNFLTEDQIL WLLLGICRGL EAIHAKGYAH RDLKPTNILL GDEGQPVLMD LGSMNQACIH VEGSRQALTL QDWAAQRCTI SYRAPELFSV QSHCVIDERT DVWSLGCVLY AMMFGEGPYD MVFQKGDSVA LAVQNQLSIP QSPRHSSALR QLLNSMMTVD PHQRPHIPLL LSQLEALQPP APGQHTTQI
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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