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Stop Solution for TMB Substrate

Stop Solution for TMB Substrate

Cat no: 423001

Supplier: BioLegend
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Biolegend’s Stop Solution for TMB Substrate is specifically formulated to terminate the HRP/TMB reaction and stabilizing the color development for ELISA applications. TMB is a chromogenic substrate used in ELISA. TMB can act as a proton donor for the reduction of hydrogen peroxide to water by peroxidase enzymes such as HRP. The resulting diimine causes the solution to take on a blue color, and this color change can be read at 650 nm. The reaction can be stopped by addition of stop reagent, and the color turns from blue to yellow with 2-3 fold increased absorbance values at 450 nm.
Catalogue number: 423001
Applications: ELISA
Size: 60 ml
Storage buffer: Store at room temperature.
Regulatory status: RUO
Shipping temp: Ambient RT
Storage temp: Ambient RT
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