Streptavidin (Lyophilized, Preservative Free) (HRP)

Streptavidin is a non-glycosylated polypeptide chain having a molecular mass of 240kD and containg 4 subunits each with a binding site for biotin. The neutral isoelectric point of Streptavidin and the high affinity of the biotin-streptavidin interaction result in a very quick reaction with a high signal to noise ratio. Streptavidin does not react non-specifically with endogenous lectins when used in immunological assays on cells and tissues. Streptavidin is non- or very low charged around pH 7, reducing NSB to charged molecules to a very limited level.\n\nApplications:\nSuitable for use in ELISA, Immunoblot (Western and Dot Blot), Immunohistochemistry and Immunoperoxidase Electron Microscopy. Other applications not tested.\n\nRecomended Dilution:\nELISA: 1-2ug/ml\n\nWestern Blot: \n0.01-0.1ug/ml for Western blotting with ECL substrates\n1-2ug/ml for Western blotting with chromogenic substrates\n\nImmunohistochemistry: 1-2ug/ml\nOptimal dilutions to be determined by the researcher.\n\nReconstitution and Storage: Store freeze-dried product at 2-8 degrees C until reconstitution. When ready to use, reconstitute with 1 ml di water. Centrifuge product if the solution is not clear after reconstitution. To judge clarity, draw product into a clean pasteur pipette. Storing the product for more than one day at final working dilutions is not recommended. For extended storage as a liquid, add an equal volume of glycerol (ACS or better grade) for a final concentration of 50%\nfollowed by storage at -20 degrees C. Please note that the concentration of protein and buffer salts will be one-half of the original after the addition of glycerol. Alternatively, the product may be aliquoted and frozen at -70 degrees C or below, in the absence of glycerol. It is important to avoid repeated freezing and thawing. \n\nExpiration Date: one year from date of reconstitution.\n\nCountry of Origin: USA\n

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SPECIFICATIONS

Catalog Number

S7973-97D

Size

1mg

Applications

ELISA, IHC, WB

Form

Supplied as a lyophilized powder in 0.01M sodium phosphate, 0.25M sodium chloride, pH 7.6, 15mg/ml BSA (IgG and Protease Free). No preservative added. Labeled with horseradish peroxidase (HRP). (Note: Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.)

Purity

Purified by ion exchange chromatography.

Additional Info

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase and anti-streptavidin. No reaction was observed against anti-avidin.