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Streptavidin (PE)

Cat no: S7973-92F2

Streptavidin (PE)

Streptavidin labeled with PE.\n\nPreparation:\nPure Streptavidin is reacted with Succinimidyl 4- (N-maleimido-methyl) cyclohexane-1- carboxylate (SMCC). R. Phycoerythrin is reacted with N-succinimidyl 3-(2-pyridyldithio) propionate (SPDP) and activated by reduction with dithiothreitol. The two substitiuted proteins are reacted together to give covalent conjugates, which are selected by medium pressure liquid chromatography on gel filtration columns of AcA34 ultrogel. Conjugates show negligible non-specific binding to non-biotinylated macromolecules, and therefore gives very low backgrounds. \n\nApplications: Suitable for use in Flow Cytometry. Other applications not tested.\n\nRecommended Dilutions:\nFlow Cytometry: Neat. Use 10ul to label 10e6 cells in 100ul. \nOptimal working dilutions to be determined by researcher.\n\nStorage and Stability: Stored at 4 degrees C. Do not freeze. Protect from light. Aliquots are stable for at least 12-12 months at 4 degrees C

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SPECIFICATIONS

Catalog Number

S7973-92F2

Size

100Tests

Applications

FC

Form

Supplied as a lyophilized powder in PBS, 0.1% sodium azide, 1% BSA. Labeled with Phycoerythrin (PE). Reconstitute with 1ml sterile dH2O.

Purity

Purified by medium pressure liquid chromatography on gel filtration columns of AcA34 ultrogel.

Additional Info

Demonstrates negligible non-specific binding to non-biotinylated macromolecules. Gives very low backgrounds.

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