Streptavidin

The Streptavidin preparation contains an N- and C-terminal shortened variant (core streptavidin) with improved properties concerning homogeneity, solubility, resistance towards proteolytic degradation and accessibility of the biotin binding pocket as compared to native streptavidin. Streptavidin has a molecular weight of 53kD.\n\nSpecific Activity: >13 U/mg (1unit binds 1ug biotin)\n\nProteolytic Activity: <10-3 U/mg protein (Azocoll, 25 degrees C, 24 h, pH 8.0).\n\nApplication: \nAvidin may be used to visualize biotin conjugated molecules in ELISA, blotting and histological techniques.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Powder is stable for 12 months at 20 degrees C. For longer storage in dissolved form add 1mM EDTA and/or 0.02 % NaN3 or pass the solution through a sterile filter. Prevent freeze-thaw cycles. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S7973-75A

Size

5mg

Form

Supplied as a sterile-filtered lyophilized powder in 10mM potassium phosphate buffer, pH 6.5. Gives a clear solution at 5mg/ml in 0.1M NaCl.

Purity

(same/more than) 95% by SDS-PAGE

References

1. Sensitivity enhancement of DNA microarray on nano-scale controlled surface by using a streptavidin-fluorophore conjugate.\n\nBiosens Bioelectron 2006 Jun 27;\n\n2. AHNP-streptavidin: a tetrameric bacterially produced antibody surrogate fusion protein against p185(her2/neu).\n\nOncogene 2006 Jun 19;\n\n3. Detection of anti-EPO antibodies in human sera by a bridging ELISA is much more sensitive when coating biotinylated rhEPO to streptavidin rather than using direct coating of rhEPO.\n\nJ Immunol Methods 2006 May 19;\n\n4. Synthesis of a biotin derivative of iberiotoxin: binding interactions with streptavidin and the BK Ca2+-activated K+ channel expressed in a human cell line.\n\nBioconjug Chem 2006 May-Jun;17(3):689-99\n\n5. The high-resolution structure of (+)-epi-biotin bound to streptavidin.\n\nActa Crystallogr D Biol Crystallogr 2006 Jun;62(Pt 6):576-81\n\n6. Dendritic-like streptavidin/alkaline phosphatase nanoarchitectures for amplified electrochemical sensing of DNA sequences.\n\nLangmuir 2006 Apr 25;22(9):4305-9