Streptomycin Sulfate is a broad spectrum antibiotic used in mammalian cell culture. It belongs to a group of drugs called aminoglycosides. Streptomycin inhibits prokaryote protein synthesis by binding to S12 protein of 30S ribosome. It prevents the transition from initiation complex to chain-elongating ribosome and causes miscoding. Thus, it prevents bacteria from manufacturing their cell walls.
Potency:
650-850u/mg
Appearance: White crystalline powder
Identification: Conforms to USP
pH: 4.5-7.0
Loss on Drying: (same/less than)5%
Sterility: Conforms to USP
Streptomycin B: (same/less than)3%
Sulfated Ash: (same/less than)1%
Sulfate: 18.0-21.5% (anhydrous)
Sodium Metabisulphite:
(same/less than)0.3%
Endotoxin: (same/less than)0.25EU/mg
Storage and Stability:
Reconstitute by adding sterile buffer. Aliquot and store at -20 degrees C. Reconstituted product is stable for 3 days at 37 degrees C; up to one month at 4 degrees C; 6-12 months at -20 degrees C. Further dilutions can be made in assay buffer.
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