Stromal Cell-Derived Factor-1 alpha, Recombinant, Human (SDF1A, Stromal Cell-derived Factor 1, SDF1, SDF-1, hSDF-1, Chemokine (C-X-C motif) Ligand 12, C-X-C Motif Chemokine 12, CXCL12, Intercrine Reduced in Hepatomas, IRH, hIRH, Pre-B Cell Growth-stimulat

Source:\nRecombinant corresponding to 68aa of human Stromal Cell-Derived Factor-1 alpha expressed in E.coli is a non-glycosylated, polypeptide chain.\n\nMolecular Weight: \n~8kD\n\nBiological Activity:\nThe specific activity as determined by its ability to chemoattract human peripheral T cells activated with PHA and IL-2 using a concentation of 20-80nug/ml.\n\nDimers and Aggregates:\n(same/less than)1% (SDS-PAGE)\n\nProtein Content:\nProtein quantitation was carried out by two independent methods: 1.UV spectroscopy at 280nm using the absorbency value of 1.06 as the extinctioncoefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENEcomputer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of SDF-1 as a Reference Standard.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O, HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S7975-50E

Size

2ug

Form

Supplied as a lyophilized powder. No preservative added. Reconstitute with sterile dH2O, 0.1% HSA or BSA to (same/more than)0.1mg/ml.

Purity

~98% (RP-HPLC, Anion-exchange FPLC, SDS-PAGE) Endotoxin: (same/less than)0.1ng/ug (IEU/ug)

References

1. Chemokine receptor CXCR4-dependent internalization and resecretion of functional chemokine SDF-1 by bone marrow endothelial and stromal cells. Nat Immunol 2005 Oct;6(10):1038-46. 2. Complement C3a enhances CXCL12 (SDF-1)-mediated chemotaxis of bone marrow hematopoietic cells independently of C3a receptor. J Immunol 2005 Sep 15;175(6):3698-706. 3. RGS16 is a negative regulator of SDF-1-CXCR4 signaling in megakaryocytes. Blood 2005 Nov 1;106(9):2962-8. 4. Causal pathways of the effects of age and the CCR5-Delta32, CCR2-64I, and SDF-1 3'A alleles on AIDS development. J Acquir Immune Defic Syndr 2005 Jul 1;39(3):321-6. 5. Stromal cell-derived factor-1 (SDF-1) alleles and susceptibility to breast carcinoma. Cancer Lett 2005 Jul 28;225(2):261-6. 6. Trafficking of normal stem cells and metastasis of cancer stem cells involve similar mechanisms: pivotal role of the SDF-1-CXCR4 axis.\nStem Cells 2005 Aug;23(7):879-94.