Stromal Cell Derived Factor 1 alpha, Recombinant, Mouse (SDF1a)

Recombinant Murine SDF-1a produced in E. coli is a non-glycosylated, polypeptide chain containing 68 amino acids and having a molecular mass of 7921 Dalton..\n\nBiological Activity: SDF-1alpha is fully biologically active when compared to standard. The specific activity as determined by its ability to chemoattract human monocytes at 50-100ng/ml ml.\n\nAA Sequence:\nKPVSLSYRCP CRFFESHIAR ANVKHLKILN TPNCALQIVA RLKNNNRQVC IDPKLKWIQE YLEKALNK \n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O, aliquot and store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S7975-51C

Size

2ug

Form

Supplied as a lyophilized powder. Reconstitute with sterile dH2O PBS to 0.1-1mg/ml, which can be further diluted into other aqueous solutions.

Purity

(same/more than) 98% as determined by RP-HPLC; Reducing and non-reducing SDS-PAGE.

References

1. Haplotype-independent costimulation of IL-10 secretion by SDF-1/CXCL12 proceeds via AP-1 binding to the human IL-10 promoter. J Immunol 2007 Feb 1;178(3):1581-8\n2. The pivotal role of CXCL12 (SDF-1)/CXCR4 axis in bone metastasis. Cancer Metastasis Rev 2006 Dec;25(4):573-87\n3. Expression of SDF-1 and CXCR4 during reorganization of the postnatal dentate gyrus. Dev Neurosci 2007;29(1-2):48-58\n4. Functional CXCR4-expressing microparticles and SDF-1 correlate with circulating acute myelogenous leukemia cells. Cancer Res 2006 Nov 15;66(22):11013-20\n5. p53 Attenuates cancer cell migration and invasion through repression of SDF-1/CXCL12 expression in stromal fibroblasts. Cancer Res 2006 Nov 15;66(22):10671-6\n6. SDF-1 expression is elevated in chronic human renal allograft rejection. Clin Transplant 2006 Nov-Dec;20(6):712-8