Substance P Receptor (NK-1 Receptor)

The tachykinins belong to an evolutionary conserved family of peptide neurotransmitters that share the C-terminal sequence Phe-X-Gly-Leu-Met-NH2 and have an established role in neurotransmission. The mammalian tachykinins include substance P, neurokinin A (NKA) and neurokinin B (NKB) which exert their effects by binding to specific receptors. Tachykinin peptides are important in the mediation of many physiological and pathological processes including inflammation, pain, migraine headache and allergy induced asthma. Three tachykinin receptor types have been characterized, NK-1, NK-2 and NK-3 which have preferential affinities for SP, NKA and NKB respectively. All three receptors share a high degree of sequence homology, have seven transmembrane spanning domains and similar signal transduction mechanisms (e.g. G-protein coupled activation of phospholipase C).\n\nApplications: \nSuitable for use in Western Blot, Immunofluorescence and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nImmunohistochemistry (Frozen): 1:1000. Can also be used on fixed tissue sections. In immunohistochemical experiments, plasma membrane staining is observed.\nImmunofluorescence (Immunocytochemistry): 1:50 \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

S8000-51B

Size

100ul

Applications

ICC, IF, IHC, WB

Hosts

Rabbit

Reactivities

Mouse, Rat, GP

Form

Supplied as a liquid.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Broccardo M, et al. Am J Physiol Gastrointest Liver Physiol 291(3):G518-24 (2006). 2. Commons KG, et al. Neuropsychopharmacology 28(2):206-15 (2003). 3. Mounir S, et al. Neurosci Res 44(1):71-81 (2002). 4. Ma MC, et al. Am J Physiol Renal Physiol 283(1):F164-72 (2002). 5. Talman WT, et al. Cell Mol Neurobiol. 2012 Apr 8. [PMID: 22484855] (IHC-Fr, IF, Rat). 6. Caioli S, et al. Neurobiol Dis. 44(1):92-101 7 (2011). Yip J, Chahl LA. Regul Pept 98(1-2):55-62 (2001). 8. Setou M, et al. Science 288(5472):1796-802 (2000). 9. Grip L, et al. Substance P alterations in skin and brain of chronically stressed atopic-like mice. J Eur Acad Dermatol Venereol. 2012 Jan 18. [PMID: 22251186] (IHC, IF, Mouse). 10. Kc P, et al. Respir Physiol Neurobiol 133(1-2):75-88 (2002). 11. Anderson LE, Seybold VS. Neurosci Lett 283(1):29-32 (2000).

Additional Info

Recognizes the rat Substance P Receptor. Species Crossreactivity: mouse and guinea pig.