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SUFU, Recombinant, Human, aa1-484, His-tag (Suppressor of Fused Homolog, SUFUH, UNQ650/PRO1280)

Cat no: S8072-01U

SUFU, Recombinant, Human, aa1-484, His-tag (Suppressor of Fused Homolog, SUFUH, UNQ650/PRO1280)

SUFU (Suppressor of fused homolog), belongs to the SUFU family. This protein is negative regulator of the hedgehog signaling pathway. Defects in this gene are a cause of medulloblastoma. SUFU has been shown to interact with GLI1, GLI3 and PEX26.\n\nSource:\nRecombinant corresponding to aa1-484 from human SUFU, fused to His-tag at N-terminal expressed in E.coli.\n\nAA Sequence:\nMGSSHHHHHH SSGLVPRGSH MAELRPSGAP GPTAPPAPGP TAPPAFASLF PPGLHAIYGE CRRLYPDQPN PLQVTAIVKY WLGGPDPLDY VSMYRNVGSP SANIPEHWHY ISFGLSDLYG DNRVHEFTGT DGPSGFGFEL TFRLKRETGE SAPPTWPAEL MQGLARYVFQ SENTFCSGDH VSWHSPLDNS ESRIQHMLLT EDPQMQPVQT PFGVVTFLQI VGVCTEELHS AQQWNGQGIL ELLRTVPIAG GPWLITDMRR GETIFEIDPH LQERVDKGIE TDGSNLSGVS AKCAWDDLSR PPEDDEDSRS ICIGTQPRRL SGKDTEQIRE TLRRGLEINS KPVLPPINPQ RQNGLAHDRA PSRKDSLESD SSTAIIPHEL IRTRQLESVH LKFNQESGAL IPLCLRGRLL HGRHFTYKSI TGDMAITFVS TGVEGAFATE EHPYAAHGPW LQILLTEEFV EKMLEDLEDL TSPEEFKLPK EYSWPEKKLK VSILPDVVFD SPLH\n\nMolecular Weight:\n~56.1kD\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S8072-01U

Size

100ug

Form

Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, 2mM DTT, 10% glycerol, 50mM sodium chloride.

Purity

~90% (SDS-PAGE)

References

1. Dunaeva M, et al. (2003) J. Biol. Chem. 278 (7): 5116-22. 2. Murone M., et al. (2000) Nat. Cell Biol. 2:310-312

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