Home  >  Products  >  SUMF1, Recombinant, Human, aa91-374, His-tag (Sulfatase-modifying Factor 1, C-alpha-formylglycine-generating Enzyme 1, FGE, PSEC0152, UNQ3037/PRO9852)

SUMF1, Recombinant, Human, aa91-374, His-tag (Sulfatase-modifying Factor 1, C-alpha-formylglycine-generating Enzyme 1, FGE, PSEC0152, UNQ3037/PRO9852)

Cat no: 029703


Supplier: United States Biological
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SUMF1 (Sulfatase-modifying factor 1) belongs to the SUMF family. SUMF1 is an enzyme that catalyzes the hydrolysis of sulfate esters by oxidizing a cysteine residue in the substrate sulfatase to an active site 3-oxoalanine residue, which is also known as C-alpha-formylglycine. Mutations in this gene cause multiple sulfatase deficiency, a lysosomal storage disorder. Source: Recombinant corresponding to aa91-374 from human SUMF1, fused to His-tag at N-terminal expressed in E.coli. AA Sequence: MGSSHHHHHH SSGLVPRGSH MVPIPAGVFT MGTDDPQIKQ DGEAPARRVT IDAFYMDAYE VSNTEFEKFV NSTGYLTEAE KFGDSFVFEG MLSEQVKTNI QQAVAAAPWW LPVKGANWRH PEGPDSTILH RPDHPVLHVS WNDAVAYCTW AGKRLPTEAE WEYSCRGGLH NRLFPWGNKL QPKGQHYANI WQGEFPVTNT GEDGFQGTAP VDAFPPNGYG LYNIVGNAWE WTSDWWTVHH SVEETLNPKG PPSGKDRVKK GGSYMCHRSY CYRYRCAARS QNTPDSSASN LGFRCAADRL PTMD Molecular Weight: ~34.1kD Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: 029703
Size: 50ug
Form: Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 2M urea, 20% glycerol, 2mM DTT.
Purity: ~85% (SDS-PAGE)
References: 1. Fraldi A., et al. (2007) Biochem J. 403(2):305-12. 2. Cosma MP., et al. (2003) Cell 113(4):445.

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