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Superoxide Dismutase, Mn, aa25-222, Recombinant, Human (Manganese Superoxide Dismutase, Manganese SOD, Mn SOD, MnSOD, IPO-B, Superoxide Dismutase [Mn] Mitochondrial Precursor, Superoxide Dismutase 2 Mitochondrial, SOD 2, SOD2, SOD-2)

Cat no: S8060-25J

Superoxide Dismutase, Mn, aa25-222, Recombinant, Human (Manganese Superoxide Dismutase, Manganese SOD, Mn SOD, MnSOD, IPO-B, Superoxide Dismutase [Mn] Mitochondrial Precursor, Superoxide Dismutase 2 Mitochondrial, SOD 2, SOD2, SOD-2)

SOD2 is a member of the iron/manganese superoxide dismutase family. It is a mitochondrial matrix protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this protein have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Recombinant human SOD2 protein was expressed in E. coli.\n\nSource: \nRecombinant corresponding to aa25-222 of human SOD2 6x His tagged expressed in E. coli.\n\nAA Sequence:\nMGSSHHHHHH SSGLVPRGSH MKHSLPDLPY DYGALEPHIN AQIMQLHHSK HHAAYVNNLN VTEEKYQEAL AKGDVTAQIA LQPALKFNGG GHINHSIFWT NLSPNGGGEP KGELLEAIKR DFGSFDKFKE KLTAASVGVQ GSGWGWLGFN KERGHLQIAA CPNQDPLQGT TGLIPLLGID \nVWEHAYYLQY KNVRPDYLKA IWNVINWENV TERYMACKK\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

S8060-25J

Size

100ug

Applications

WB

Form

Supplied as a liquid in 20mM Tris HCl, pH 8.0, 20% glycerol.

Purity

~ 95% (SDS-PAGE). Purified by using conventional chromatography techniques.

References

1. MacMillan-Crow L.A., et al. (1999) Arch. Biochem. Biophys. 366:82-88 2. Prunotto M, et al. (2010) J Am Soc Nephrol. 21(3):507-19.

Alternative Names

EC=1.15.1.1, MVCD6

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