Survivin (Apoptosis Inhibitor 4, Apoptosis Inhibitor-4, API4, Apoptosis Inhibitor Survivin, Baculoviral IAP Repeat-containing 5, BIRC5, BIRC-5, EPR1, EPR-1, IAP4, SVV, TIAP)

Regulated inhibition of programmed cell death (apoptosis) preserves normal homeostasis and tissue and organ morphogenesis. Aberrations in this process contribute to human diseases and cancer by abnormally prolonging cell viability. Recently, several apoptosis inhibitors related to the baculovirus iap gene have been found in various species, including human. IAP proteins contain one/three Cys/His baculovirus IAP repeats plus a c-terminus RING finger and are thought to block an evolutionary conserved step in apoptosis.\nSurvivin encodes a structurally unique inhibitor of apoptosis (IAP). Survivin expression is turned off during fetal development and is not found in non-neoplastic adult human tissues. Survivin becomes abundantly re-expressed in transformed cells and in all of the most common cancers of lung, colon, pancreas, breast and prostate in vivo. Survivin appears to be situated at the crossroads of cell death and cell division, governing a checkpoint involved in cytokinesis while also suppressing apoptosis.\nSurvivin is also abundantly expressed in brain tissues (astrocytes and some neurons) of adult rats following traumatic brain injury. Survivin has been found co-expressed with NeuN (mature neuronal marker) and PCNA (a cell cycle protein). Survivin might affect regulation of neural cell proliferative responses after brain injury.\n\nApplications: \nSuitable for use in Immunofluorescence, Western Blot, Immunohistochemistry (paraffin-embedded sections) and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S8500-01M

Size

100ul

Applications

ICC, IF, IHC, WB

Hosts

Mouse

Reactivities

Hum

Form

As reported

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1,k

References

1. G. Ambrosini et al. Induction of Apoptosis and Inhibition of Cell Proliferation by survivin Gene Targeting. JBC, 273(18): 11177-11182, 1998. \n2. F. Li et al. Pleiotropic cell-division defects and apoptosis induced by interference with survivin function. Nature Cell Biology, 1(8): 461-466, 1999. \n3. D. Grossman et al. Expression and targeting of the apoptosis inhibitor, survivin, in human melanoma. J. of Invest Dermatology, 113(6): 1076-81, 1999.\n4. F. Li, et al. Control of apoptosis and mitotic spindle checkpoints by survivin. Nature. 396: 580-584, 1998.\n5. D. Grossman, et al. Expression and Targeting of the Apoptosis Inhibitor, Survivin, in Human Melanoma. J. Invest. Dermatology. 113: 1076-1081, 1999.\n6. C. Adida, et al. Prognostic significance of survivin expression in diffuse large B-cell lymphomas. Blood. 96(5): 1921-1925, 2000.

Additional Info

Specific for the nuclear form of human survivin. The epitope recognized is between amino acids 3-19. Other species have not been tested.