Survivin, Human (Surv, Apoptosis Inhibitor Survivin, BIRC5, API4) BioAssay(TM) ELISA Kit

Sandwich ELISA quantitative detection kit for Human Survivin in cell culture supernates, serum and plasma (heparin, EDTA).\n\nSurvivin (also termed API4) belongs to the family of genes known as inhibitors of apoptosis, and it has been implicated in both prevention of cell death and control of mitosis. Survivin is prominently expressed in transformed cell lines and in all the most common human cancers of lung, colon, pancreas, prostate and breast in vivo. It is also found in approximately 50% of high-grade non-Hodgkin's lymphomas. Survivin encodes a deduced 142-amino acid protein and localizes on chromosome 17q25. The overexpression of it in cancer may overcome an apoptotic checkpoint and favour aberrant progression of transformed cells through mitosis. The standard product used in this kit is recombinant human Survivin with the molecular mass of 34kD.\n\nAssay Range:\n62.5pg/ml-4000pg/ml\n\nSensitivity:\n< 2 pg/ml\n\nCrossreactivity:\nThere is no detectable cross-reactivity with other relevant proteins.\n\nUniprot ID:\nO15392\n\nEntrez Gene ID:\n332\n\nKit Components:\n96-well plate pre-coated with anti-Survivin, Human\nLyophilized recombinant Survivin, Human standard, 10ng/tube, 2Ea\nUSB Cat No Kit Component\n143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution)\n143697 Sample diluent buffer, 30ml\n143698 Antibody diluent buffer, 12ml\n143699 ABC diluent buffer, 12ml\n143700 TMB color developing agent, 10ml\n143701 TMB stop solution, 10ml\n\nStorage and Handling:\nStore at 4 degrees C for 6 months, at -20 degrees C for 12 months. Avoid multiple freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

144046

Size

96Tests

References

1. Blanc-Brude, O. P.; Yu, J.; Simosa, H.; Conte, M. S.; Sessa, W. C.; Altieri, D. C. : Inhibitor of apoptosis protein survivin regulates vascular injury. Nature Med. 8: 987-994, 2002.\n2. Ambrosini, G.; Adida, C.; Altieri, D. C. : A novel anti-apoptosis gene, survivin, expressed in cancer and lymphoma. Nature Med. 3: 917-921, 1997.\n3. Li, F.; Ambrosini, G.; Chu, E. Y.; Plescia, J.; Tognin, S.; Marchisio, P. C.; Altieri, D. C. : Control of apoptosis and\nmitotic spindle checkpoint by survivin. Nature 396: 580-584, 1998.\n