Synaptotagmin-12 (P65, SVP65, DKFZp781D2042, SYT,SRG1)

Synaptotagmins constitute a family of membrane-trafficking proteins that are characterized by an N-terminal transmembrane region (TMR), a variable linker, and two C-terminal C2 domains - C2A and C2B. There are 15 members in the mammalian synaptotagmin family. There are several C2-domain containing protein families that are related to synaptotagmins, including\ntransmembrane (Ferlins, E-Syts, and MCTPs) and soluble RIMs, Munc13s, synaptotagmin-related proteins and B/K) proteins.\n\nThe synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin participates in triggering neurotransmitter release at the synapse. The first domain mediates Ca(2+)-dependent phospholipid binding. The second C2 domain mediates interaction with Stonin 2.\n\nSynaptotagmin may have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated\nprotein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2 (1, 2).\n\nApplications: \nSuitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\n1ug/ml was sufficient for detection of Synaptotagmin-12 in 20ug of transiently overexpressing synaptotagmin-12 COS cell lysate by Western Blot using Goat anti-mouse IgG:HRP as the secondary antibody.\nWestern Blot: 1:1000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

167608

Size

100ug

Applications

ICC, IHC, WB

Hosts

Mouse

Reactivities

Mouse, Rat

Form

Supplied as a liquid in PBS pH7.4, 50% glycerol.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Schengrund C.L., et al. (2002) J Biol Chem. 277 32815.\n2. Reichardt L.F., et al. (1981) J Cell Biol. 91:257.

Additional Info

Recognizes mouse Synaptotagmin-12. Species Crossreactivity: Rat, Mouse. Detects ~45kD. Does not cross-react with other Synaptotagmin-6 or other Synaptotagmins.