Synuclein, gamma, Recombinant, Human (BCSG1, SYN)

gamma-synuclein(Originally known as a breast cancer specific gene product, BCSG1) is an acidic neuronal protein of 127 amino acids. The protein coding region of gamma-synuclein was amplified by RT-PCR and cloned into an E.coli expression vector. g-synuclein was overexpressed in E. coli and purified to apparent homogeneity by taking advantage of the thermosolubility of the protein and by using conventional column chromatography techniques.\n\nDescription: \nRecombinant Human Gamma-Synuclein produced in E.Coli is a single, non-glycosylated polypeptide chain containing 127 amino acids and having a molecular mass of 13.3kD.\n\nSequence: \nMDVFKKGFSI AKEGVVGAVE KTKQGVTEAA EKTKEGVMYV GAKTKENVVQ SVTSVAEKTK EQANAVSEAV VSSVNTVATK TVEEAENIAV TSGVVRKEDL RPSAPQQEGV ASKEKEEVAE EAQSGGD\n\nStorage and Stability: \nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid mltiple freeze-thaw cycles..

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SPECIFICATIONS

Catalog Number

S9502-15A

Size

20ug

Form

Supplied as a liquid in 20mM Tris-HCl, pH 7.5, 0.1M NaCl and 1mM MgCl2.

Purity

(same/more than) 95% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.

References

1. gamma-synuclein has a dynamic intracellular localization.\n\nCell Motil Cytoskeleton 2006 May 26;\n\n2. Secondary structure and dynamics of micelle bound beta- and gamma-synuclein.\n\nProtein Sci 2006 May;15(5):1162-74\n\n3. Interaction of myocilin with gamma-synuclein affects its secretion and aggregation.\n\nCell Mol Neurobiol 2005 Sep;25(6):1009-33\n\n4. Subcellular localisation of recombinant alpha- and gamma-synuclein.\n\nMol Cell Neurosci 2005 Feb;28(2):326-34\n\n5. Different levels of gamma-synuclein mRNA in the cerebral cortex of dominant, neutral and submissive rats selected in the competition test.\n\nGenes Brain Behav 200 5 Feb;4(1):60-4\n\n6. Altered expression of gamma-synuclein and detoxification-related genes in lungs of rats exposed to JP-8.\n\nAm J Respir Cell Mol Biol 2005 Mar;32(3):192-200