SYTL5 (Synaptotagmin-like Protein 5, SLP5)

SYTL5 belongs to the synaptotagmin-like (Slp) protein family, which contains a unique Slp homolog domain (SHD) at the N-terminus. The SHD functions as a binding site for Rab27A, which plays a role in protein transport. Expression of SYTL5 is restricted to placenta and liver, suggesting that it might be involved in Rab27A-dependent membrane trafficking in specific tissues. Several members of the synaptotagmin family are calcium-sensing vesicle-fusion proteins as mediators of cell migration. SYTL7 & SYTL5 were positive regulators of chemotaxis, whereas SYTL2 was a negative regulator of chemotaxis.\n\nApplications:\nSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-2ug/ml\nImmunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nHeLa Cell Lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

146874

Size

100ug

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Kuroda TS, Fukuda M, Ariga H, et al. Synaptotagmin-like protein 5: a novel Rab27A effector with C-terminal tandem C2 domains. Biochem. Biophys. Res. Commun. 2002; 293:899-906. 2. Saxena SK, Horiuchi H, and Fukuda M. Rab27a regulates epithelial sodium channel (ENaC) activity through synaptotagmin-like protein (SLP-5) and Munc13-4 effector mechanism. Biochem. Biophys. Res. Commun. 2006; 344:651-7. 3. Colvin RA, Means TK, Diefenbach TJ, et al. Synaptotagmin-mediated vesicle fusion regulates cell migration. Nat. Immunol. 2010; 11:495-502 4. Fukuda M, Mikoshiba K. Synaptotagmin-like protein 1-3: a novel family of C-terminal-type tandem C2 proteins. Biochem. Biophys. Res. Commun. 2001; 281:1226-33.

Additional Info

Recognizes human SYTL5. Species Crossreactivity: mouse and rat.