Established from a lung metastasis of colon carcinoma in a 72 year old male. Tumour tissue was inoculated sub-cutaneously and serially transplanted in BALB/c nude mice and subsequently established in in vitro culture. The histological characteristics of the tumour were maintained throughout the transplantation procedure. They have receptors for a range of peptide hormones and neurotransmitters. The cells grow as monolayers, exhibit tight junctions and desmosomes between adjacent cells. The cells should be maintained at high density (at least 1/4 confluency) and are positive for keratin by immunoperoxidase staining.
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