T-box Protein 21 (T-box Transcription Factor TBX21, TBX21, T-cell-specific T-box Transcription Factor T-bet, TBET, T-bet, T-PET, Transcription Factor TBLYM, TBLYM)

The T-box gene family consists of transcription factors characterized by a related DNA-binding domain (T-box) of approximately 200aas (1,2). The T-box genes exhibit diverse temporal and spatial patterns in the developing embryo. Studies have demonstrated members\nof this family play crucial roles during embryogenesis in a wide range of organisms by regulating cell fate decisions to establish the early body plan and to regulate later processes underlying organogenesis (3-5). Mutations in T-box genes are associated with many developmental defects (6). Recent studies also indicate potential roles in cancer by members of T-box family (7-9).\nTBX21, also as known as T-bet, plays a critical role in development and maintenance of type 1 helper T (Th1) and TBX21 deficient mice display impaired Th1 differentiation (10,11).\n\nApplications: \nSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4 degrees C with gentle shaking, overnight.\nImmunoprecipitation: 1:50\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

T1848-50

Size

100ul

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

(1) Wilkinson, D.G. et al. (1990) Nature 343, 657-9. (2) Papaioannou, V.E. and Silver, L.M. (1998) Bioessays 20,9-19. (3) Showell, C. et al. (2004) Dev Dyn 229, 201-18. (4) Papaioannou, V.E. (2001) Int Rev Cytol 207, 1-70. (5) Hoogaars, W.M. et al. (2007) Cell Mol Life Sci 64, 646-60. (6) Baldini, A. (2004) Curr Opin Cardiol 19, 201-4. (7) Abrahams, A. et al. ( 2010) IUBMB Life 62, 92-102. (8) Rowley, M. et al. (2004) J Mammary Gland Biol Neoplasia 9,109-18. (9) Yang, X.R. et al. (2009) Nat Genet 41, 1176-8. (10) Ho, I.C. and Glimcher, L.H. (2002) Cell 109 Suppl, S109-20. (11) Peng, S.L. (2006) Cell Mol Immunol 3, 87-95.

Additional Info

Recognizes endogenous levels of total TBX21.