T-Complex Protein 1 alpha (T Complex Protein 1 alpha Subunit, T Complex Protein 1 Subunit alpha, TCP1 alpha, TCP1-alpha, TCP-1 alpha, CCT 1, CCT1, CCT alpha, CCTa, D6S230E, T Complex 1, T-complex 1, T Complex Locus TCP1, T-complex Locus TCP-1, T Complex P

Applications: \nSuitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: RK13 heat shocked cell lysate.\n\nCellular Localization: Cytoplasmic\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 3 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T2035-02C

Size

100ug

Applications

ICC, IP, WB

Hosts

Rat

Reactivities

Hum, Mouse, Rat, Bov, Can, Rabbit, Sheep

Form

Supplied as a liquid in PBS, pH 7.2. No preservative added.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2c

References

Sternlicht H et al. The t-complex polypeptide 1 complex is a chaperonin for tubulin and actin in vivo. Proc Natl Acad Sci U S A 90:9422-6 (1993). <PUBMED:8105476>Lewis VA et al. T-complex polypeptide-1 is a subunit of a heteromeric particle in the eukaryotic cytosol. Nature 358:249-52 (1992). <PUBMED:1630492>Willison K et al. The t complex polypeptide 1 (TCP-1) is associated with the cytoplasmic aspect of Golgi membranes. Cell 57:621-32 (1989). <PUBMED:2655925>Willison KR et al. Molecular cloning and sequence analysis of a haploid expressed gene encoding t complex polypeptide 1. Cell 44:727-38 (1986). <PUBMED:3753900>

Additional Info

This antibody recognizes other proteins, most notably the p102B' COP subunit of Golgi coatomer. It does not react with human Hsp60 protein. Species Crossreactivity: Crossreacts with Mouse, Rat, bovine, canine, Hamster, Plants, Rabbit and Sheep.