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T Lymphocytes

Cat no: T8060-01C


Supplier: United States Biological
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Applications: Suitable for use in Flow Cytometry and Immunohistochemistry (frozen and paraffin). Other applications not tested. Recommended Dilution: Flow Cytometry: 1:100-1:1,000; 10ul labels 10e6 cells in 100ul Immunohistochemistry (frozen): 1:100-1:1,000 Optimal dilutions to be determined by the researcher. Hybridoma: X63.Ag8.653 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: T8060-01C
Reactivities: Guinea Pig
Hosts: Mouse
Applications: Flow Cytometry, Immunohistochemistry
Size: 250ul
Form: Supplied as a liquid, 0.09% sodium azide, 0.7% BSA.
P type: Mab
Isotype: IgG1
Purity: Ascites
References: 1. Tan, B.T.G. et al. (1985) Production of monoclonal antibodies defining guinea pig T-cell surface markers and a strain 13 Ia like antigen: The value of immunohistological staining. Hybridoma 4: 115-124. 2. Steerenberg, P.A. et al. (1991) Tumour rejection after adoptive transfer of line-10-immune spleen cells is mediated by two T cell subpopulations. Cancer Immunol. Immunopathol. 34: 103-110. 3. Steerenberg, P.A. et al. (1991) Tumour rejection after transfer of line 10 immunity is mediated by two T-cell populations. Cancer Immunol. Immunopathol. 34: 103-110. 4. Steerenberg, P.A. et al. (1990) Tumour infiltrating leucocytes (tils) during progressive tumour growth and BCG-mediated tumour regression. Virchows Achiv B cell Pathol. 59: 185-194.
Additional info: Recognizes a cell surface antigen present on the surface of all guinea pig T lymphocytes. Weak reactivity is also seen with some myeloid cells, and with the L2C guinea pig B cell leukaemia.

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